As a part of the National Gene Bank (NGB) that follows the Agricultural Research Center of Egypt (ARC), the Microbial Genetic Resources Laboratory is interested in collection, characterization, identification, authentication and conservation the microbial genetic recourses on the national level. The Microbial Genetic Resources lab has adequate facilities and equipment for establishing microbial culture collection (MCC), in addition to conduct some research programs in biofertilizers, biological control of plant diseases and long-term preservation of microorganisms. So far, the Microbial Genetic Resources Laboratory team were managed to collect more than 400 isolates of Rhizobia From different cultivated areas with legumes. Furthermore, there is a vital concern in collecting some bacterial species such as Serratia Sp and Bacillus thuringiensis which proved lately their significant ability as powerful biological control agents.
Objectives and Strategy
Establishment of microbial culture collection (MCC) for all microorganism (bacteria, yeasts, fungi and algae).
Collection, Isolation, Phenotypic and Genotypic characterization and Identification of microorganism.
Maintenance and preservation of purified cultures using either the short-term conservation at 4°C or the long-term conservation which can be under a freeze-dried condition, frozen in glycerol at –80 °C and Cryopreservation.
Serving as a center for the national and international exchange information and microbial culture collections.
Providing public and private sectors with the required cultures for manufacturing microbial inoculants.
Action plans and the future work
Propose research plans to overcome any constraints that may encounter the microbial culture collections.
Build up database of microbial culture collections comparing all the required information concerning non-pathogenic and pathogenic microorganisms used in agriculture.
Collection, isolation, cultivation, identification, evaluation, authentication and maintenance of microorganism under adverse soil conditions in Egypt.
Improving methods of characterization, preservation and distribution of microbial germplasm collection.
Collecting and evaluating microbial strains to increase the use of microbial inoculants in agriculture.
Staff
Dr. Fayrouz Hassan Abd Elmeged +20 102 838 9552 fayrouz_1983@hotmail.com
Dr. Akram Hassan Mohamed +20 100 412 0434 akramhassan8787@gmail.com
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as Micropropagation. Tissue culture of plants refers to the growing and multiplication of plant cells tissues and organs on defined solid or liquid media for the production of high-quality uniform and disease-free plants. Moreover, tissue culture techniques are used for medium-term and long-term preservations including cryopreservation.
Objectives
Micropropagation of rare and endangered species.
Micropropagation of vegetative propagated species.
Micropropagation of recalcitrant seed-producing species.
in vitro medium-term preservation.
Long-term preservation (cryopreservation).
STAGES OF PLANT TISSUE CULTURE
Establishment stage
Multiplication stage
Rooting stage
Acclimatization stage
Staff
Prof. Dr. Neveen Abd El-Fattah Hassan +20 114 348 5555 niveen2020@hotmail.com
Dr. Ahmed Abd El-Wahab Mohamed El-Homosany +20 100 828 8309 a-homos2007@hotmail.com
Dr. Ahmed Mahmoud Haggag Abdelazim +20 114 071 4907 Ahmedhagag56@yahoo.com
The department is concerned with the genetic resources of horticultural crops presented in Egypt to preserve them from deterioration and extinction. This department includes:
Fruit crops
Vegetable crops
Medicinal and aromatic plants
Timber trees and ornamental plants
Strategy
Developing an action plan for integrated management of horticultural genetic resources targeted and cooperating with all the farms, gardens, orchards of local and regional to collect and exchange different samples of these plants.
Objectives
Survey, collection, propagation, evaluation and conservation of horticultural crops’ genetic resources, prioritizing the endemic and endangered ones.
Identification and description of the horticultural crops’ genetic resources.
Follow up and maintenance of the endangered and rare species in their natural habitat (in-situ).
Mapping, maintenance, and documentation of the endemic and near-endemic horticultural species, to preserve the national intellectual properties.
Evaluating the collected horticultural germplasms against different stresses and maximizing its nutritional, medicinal and environmental values.
Plan of Action
Carry out missions and scientific expeditions to explore the collection of plant genetic resources.
Determine the distribution and the whereabouts of plants in cooperation with the relevant authorities.
The collection of seeds and seedlings of the mind and the existing plants during trips combined with relevant.
Exchanging horticultural crop samples with other entities in Egypt and abroad. Studying the environmental conditions of different regions in the Arab Republic of Egypt to identify the optimum factors affecting plant genetic resources.
Determining the current status of the state of biodiversity in Egypt.
Training and capacity building and the establishment of specialized workshops and scientific conferences in cooperation with the competent authorities
Greenhouses
Greenhouse is a building designed with specific properties for the protection of tender or out-of-season plants against climatic variations in the open environment. On the other hand, it could be considered a perfect tool for preserving different plant genetic resources in Gene banks, especially for those species that couldn’t produce viable seeds under local conditions. So, it should be preserved as intact plants. In the Egyptian National Gene Bank (NGB), well designed and equipped greenhouse was allocated for conserving a huge number of timber trees, ornamental, medicinal and aromatic plants. Such species have been collected from all around Egypt. This germplasm collection of NGB greenhouse comprised about 83 plant families, 177 genera, and 226 species. Some of these species (wild or cultivated) are rare and endangered with high economical, medicinal and aesthetic values. The collected species including propagating materials (seeds, cuttings, bulbs,), seedlings and transplants are legally acquired with healthy condition to ensure high quality growth. After collecting and transferring to the NGB greenhouse, the collected materials are scientifically identified, documented, cultivated and supplied with different growth factors. Daily agricultural processes inside NGB are done including irrigation, controlling pests and diseases, fertilizing, etc., this is vital to ensure perfect growth of the collected plant materials. Propagation of these species is a very important process that is periodically done in the NGB greenhouse. These germplasms deposited inside NGB are available for students, researchers, gardeners, breeders, and other academic affiliations for scientific purposes as well as for exchange with new species. To ensure high performance of NGB greenhouse, growing factors (light, humidity and temperature) are completely controlled.
Botanical Garden
A small botanical garden is attached to the ENGB greenhouse, allocated for growing mature plants which become large in size and not suitable for the growth inside ENGB greenhouse. About 52 families, 134 genera and 155 species are located in the garden attached to this greenhouse.
Staff
Dr. Hossam Abd El-Maksoud Sayed Ahmed +20 112 158 5000 hosam722000yahoo.com
Dr. Tarek Mohamed Noor El-Deen Ahmed +20 100 115 1682 tarek.noor@arc.sci.eg
Genetic resources are the biological basis of global food security and directly or indirectly support human livelihoods through the food, medicine, animal feed, fibre, clothing, housing, energy and many other products and services they provide. They consist of the diversity of genetic material present in traditional varieties and modern plant stocks, as well as crop wild relatives and other natural plant species that It can be used now or in the future for food and agriculture.
Objective
Collecting and conserving plant genetic resources, from erosion and extinction.
Exchanging information concerning genetic resources with local and international gene banks.
Identifying the core collection of genetic resources that can be utilized in both public and private research and breeding programs.
Developing plans for a collection of genetic resources to ensure the safety of these resources and to provide the different breeding programs with the required genetic materials and relative information.
Characterization of the collected genetic resources.
To address the prevailing conditions of climate change for agricultural research and breeding towards achieving food security.
Strengthen the international cooperation in the field of genetic resources.
Enhancing public awareness of genetic resources maintenance to protect the national resources against erosion and to regulate the utilization of such resources.
Participating in exploration missions intended for collecting genetic resources from their native habitat.
Facilitating the exchange of genetic resources and implementation of the intellectual property legislation concerning national genetic resources.
Documenting the Egyptian genetic resources on the NGB database.
Activities
Seeds storage
In the NGB, two different forms of packing are used: plastic packing is used in both the active storage room (-5), as well as the temporary storage room (+5). while we are using specialized aluminum foil at the base storage room (-20) to preserve the samples in cold storage for an extended period.
Characterization
The description must be in the way that the breeder and the various breeding programs are benefited from; there are morphological traits with high hereditary ability that are important in breeding programs, and there are various other traits of high agricultural value that must also be recorded to characterise and identify those inputs and make full use of those genetic resources.
Notable: Characterization is carried out using international models UPOV- IPGRI.
Evaluation
It is the process of testing genetic resources by evaluating the important genetic traits carried by these germplasms that are affected by different degrees under 9 different environmental conditions such as resistance to pests and diseases or the quality of their seeds. Also, the assessment of all genetic resources for their tolerance to adverse conditions (such as tolerance to salinity – drought – heat) and vital stresses (such as: tolerance to disease, insect and pest infestation) in different regions.
Regeneration
Propagation is a major process and an integral part of the responsibility of any genebank that maintains traditional seeds. It is a process that increases the seeds (A) (B) 8 stored in the genebank and/or increases the viability of the seeds to equal or greater than the agreed minimum level. The sample is renewed if it no longer contains enough seeds for long-term storage (i.e. 1,500 seeds for self-pollinating species and 3,000 for out crossings) or when its viability falls below a specified minimum threshold (i.e., less than 85 percent of initial germination of stocked seeds). And if the demand for one of the registered samples is rare and its validity is impeccable, then the number of seeds can reach less than 1000 before renewal. Regeneration should be performed when viability drops below 85 percent of the original viability or when the amount of seed remaining is less than necessary for three cycles of the representative population of Bank-registered samples and the oldest original sample specimen should be used for regeneration of registered samples.
distribution
Gene banks aim to make available to users as many recorded samples as possible, including associated data. If stocks are depleted, samples should be multiplied to meet user demands as a matter of priority, and gene banks that maintain working collections should enhance the availability of genetic resources for uses including research, breeding, education, agriculture and reintroduction. At the international level, gene banks can be a source for providing supplies of wild origin PGR to countries that are setting up their own gene banks.
Seeds are distributed in compliance with national laws and relevant international treaties and agreements.
Submit seed samples with all relevant documents required by the recipient country
The time between receiving a seed request and sending, it is kept to a minimum
Staff
Prof. Dr. Soliman Abdelmaaboud Abdelmaaboud Arab +20 100 322 5345 Solimanngb@gmail.com
The majority of livestock integrates with crops in small holding that represent approximately 85%of its total population. the average farmer owns 1.02 buffalo cows, 0.53growing buffalo calves, 0.94 bovine cows, 0.51bovine calves, 1014 sheep 1.06 goat, number of different poultry of different species plus donkeyes and camels / or horses. The evolutionary forces of mutation, selective breeding, adaptation, isolation and genetic drift have created an enormous diversity of local populations. Livestock provide meat, milk, eggs, fibers, skins, manure for fertilizer and fuel, draught power for cultivation and transport, and a range of other products and services. Livestock biodiversity is essential to food and livelihood security, particularly in the developing world.
Animal production systems in Egypt
Semi intensive / intensive system (delta and Nile valley) mainly large ruminants, small ruminants, a number of different poultry of different species pluse donkeys and camel and / or horses.
Intensive system (poultry and dairy cattle).
Zaraba (around cities) dairy buffalo and goats.
Extensive system (Sinai, north western coastal zone, halaieb and shalatein mainly small ruminants and camels.
The states of breeds at risk
Population size of local cattle and buffalo breeds are quite stable and no indication that one of these breeds is at risk. Except Domiati cattle which could be at risk according to decrease of its population.
The major local breeds of sheep and goat do not seem at risk. The exception is zaribi breed which could be at risk as all available reports indicate deceasing of its population. The breeds of camel, horse, donkey, chicken, duck, goose, are not at risk.
Two local breeds of rabbits have already become extinct, Giza White and Baladi White and two more are endangered, Baladi Red and Baladi Black.
Main constraints facing establishment of national conservation program
Fragmented ownership of livestock.
Lack of recording of animal pedigrees / performance
Lack of applying uniform protocols for collection of germplasm and cryopreservation programs in each breed.
Lack of the accurate information about genetic characterization of our native breeds.
Lack of public understanding and awareness of the role and values of AnGR.
Lack of legislation and regulation affecting the maintenance of AnGR.
The main objective of the department
Genetic characterization of our native breeds.
Standardization of protocols for collection of germplasm, through well- defined and sound cryopreservation programs for each breed.
To rescue and increase the population of endangered breeds. (Red and Black rabbit and Zeraibi goats)
Establishing data base system for information on native breeds characterization.
This includes information on breeds population, description and qualifications.
Emphasis will be on traits related to meat quality, mothering ability and adaptation to harsh climate and poor environmental conditions.
Increasing the awareness of public for the importance of our rescue mission and to the necessity of maintaining these breeds as a gene pool for future use in rapidly changing world.
The magazine published by the Gene Bank.
Pamphlets published by Gene Bank.
Publication in the newspapers.
Publication in the agricultural magazine.
Current activity
Genetic characterization of Sohagi sheep, Barki Sheep and buffalo.
Cryopreservation studies for semen of chicken, Ducks, Turkey and Camel embryo.
Determining some genetic loci of productive traits in some indigenes chicken breeds using microsatellite markers.
4-National gene bank performing of project based on integration of genetic resources management in western desert communities including farm animal genetic resources (Genetic characterization for wild-type of cattle, sheep and goats) in four oases namely El-Kharga, El-Dakhla, El-Farafra and Siwa.
Achievements of Animal Genetic Resources Department
Genetic characterization of chicken breeds and development strains: Among Fayuomi breed (PP, GG and RR). Fayuomi breed Dandarawi breed Gimiza strain Inshas strain Doki-4 strain Golden Montazah strain Silver Montazah strain Mamorah strain Mandara strain Bahij strain Matrouh strain El-salam strain Bandarah strain Sinai strains
Determining some genetic loci of egg production traits in Fayoumi and Dandrawi chicken breeds using microsatellite markers (Ph.D. Thesis).
Variation and phylogeny studies of Egyptian River Buffalo by mitochondrial D-loop nucleotide sequences.
Semen Quality and Freezing of Sohagi Rams in Egypt
Staff
Prof. Dr. Khaled Roushdy Mohamed Mousa +20 114 331 7188 khromod@yahoo.com
Prof. Dr. Mohammed Ahmed El-sayed Hasan +20 109 966 1610 m.a.el_sayed@outlook.com
Dr. Alsayed Alsoudy Mohamed Mostafa +20 155 831 3399 alsayedalsoudy@gmail.com
Dr. Heba Abd EL-Wahab Mahmoud Assi +20 100 824 5305 Music.art723@gmail.com
The main objectives of the cytogenetics Laboratory are focused on the investigation of cytological disturbances and genetic disorders. These could be achieved through the study of:
Mitotic activity, frequency of micronuclei and the chromosomal aberrations in mitotic cell divisions as an indication of the adaptability to the Egyptian conditions in varieties, landraces and/or accessions
Meiotic irregularities, frequency of micronuclei and the chromosomal aberrations in meiotic cell divisions as an indication of the cytological instability in varieties, landraces and/or accessions. Moreover, to detect any existing association between the cytological and instabilities and agronomic characters.
Testing the pollen grain viability of different varieties, landraces and/or accessions.
Determination of chiasma frequency to test the recombination potential in varieties, landraces and/or accessions.
Investigate the chromosomal maps of wild plants, animals and landraces which can be utilized in the different breeding programs.
Study the different field and agronomic cytogenetically as related to agronomic and morphological characteristics to conclude the degree of genetic stability.
Chromosomes morphological characterization
From the karyotype analysis, the two homologues (a and b) of each chromosome pair were judged according to similarities in length of short arm, long arm and total lengths and centromeric index percentage.
Microscope examination, karyotyping and idiograming
Chromosomes examination was performed using a vertical fluorescence microscope (Leica DM2500) equipped with a cooled monochrome digital camera (Leica DFC340FX). Twenty cells with clearly observable and well spread were examined and photographed at 100 × magnification under oil immersion. Chromosome counting and karyotyping were performed using the automated karyotyping & FISH software processing (Leica CW4000) system. Idiograms were constructed from complete chromosomes which showed the maximum possible banding patterns in at least ten different metaphase plates.
Staff
Dr. Mona Ebrahim Abd El-Gawad +20 100 917 0469 monagenetist2012@gmail.com
chemical analysis Laboratory is one of the most important laboratories in the National genes Bank, the work based on the evaluation and characterization of plant genetic resources according to their chemical composition to know the importance of each genetic resource and to identify the active substances contained in it, also measuring it’s concentration by using the most accurate methods and the latest scientific equipment.
Strategy
Discuss and study the latest laboratory methods for chemical analysis used in the detection of chemical components and their application to genetic resources found in the National gene Bank.
Documentation of data and results obtained after comparing them with specialized scientific references.
Participate in the training courses for university students and new researchers to give them the necessary expertise in the field of chemical analysis and chemical classification of plant genetic resources
Objective
Determine of active substances in each plant genetic resource.
Evaluation and characterization of plant genetic resources according to their chemical composition.
Study the effect of the environment surrounding each genetic resource on the chemical composition and interpretation of the relationship between them.
Entering Data and results of the chemical analysis tests of each Plant Genetic Resource into the National gene Bank database as a reference to return to it at any time.
Provide all the information and experiences in the field of chemical analysis for all researchers and those interested in this area with the exchange of experiences between our laboratory and other laboratories specializing in this field.
The role of the chemical analysis laboratory
Knowing the chemical composition of each Plant Genetic Resource is the most important step to be taken in the process of classification and description. In this point lays the importance of chemical analysis laboratory where this role is the main point of our work.
By using the latest scientific methods and equipment The laboratory paint a picture of the estimated chemical composition of each genetic resource and describe of the differences in chemical composition between plant genetic resources and each other as the results of the relationship between the environment surrounding each resource and its chemical composition and the active substances it contains.
Staff
Prof. Dr. Abeer Ahmed Hamdy Elhalwagi +20 100 450 3095 abeerelhalwagi@gmail.com
Dr. Ali Hassan Ali Taha Anany +20 112 310 8813 ali.h_anany@yahoo.com
